Monitoring translation in synaptic fractions using a ribosome profiling strategy
نویسندگان
چکیده
منابع مشابه
Ribosome Profiling Enables Comprehensive Translation Analysis
Microarrays have the capacity to measure the expression levels of thousands of genes simultaneously1. Although microarrays have revolutionized mRNA analysis, they are only a proxy for the actual amount of protein synthesis occurring in cells. Multiple levels of transcriptional and post-transcriptional regulation contribute to the inherent challenges of proteomic analysis. A direct method for an...
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Deep sequencing of ribosome footprints (ribosome profiling) maps and quantifies mRNA translation. Because ribosomes decode mRNA every 3 nt, the periodic property of ribosome footprints could be used to identify novel translated ORFs. However, due to the limited resolution of existing methods, the 3-nt periodicity is observed mostly in a global analysis, but not in individual transcripts. Here, ...
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Ribosome profiling and high-throughput sequencing provide unprecedented opportunities for the analysis of mRNA translation. Using this novel method, several studies have demonstrated the widespread role of short upstream reading frames in translational control as well as slower elongation at the beginning of open reading frames in response to stress. Based on the initial studies, the importance...
متن کاملTranslation inhibitors cause abnormalities in ribosome profiling experiments.pptx
Ribosome profiling and high-throughput sequencing provide unprecedented opportunities for the analysis of mRNA translation. Using this novel method, several studies have demonstrated the widespread role of short upstream reading frames in translational control as well as slower elongation at the beginning of open reading frames in response to stress. Based on the initial studies, the importance...
متن کاملBayesian prediction of RNA translation from ribosome profiling
Ribosome profiling via high-throughput sequencing (ribo-seq) is a promising new technique for characterizing the occupancy of ribosomes on messenger RNA (mRNA) at base-pair resolution. The ribosome is responsible for translating mRNA into proteins, so information about its occupancy offers a detailed view of ribosome density and position which could be used to discover new translated open readi...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2020
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2019.108456